Journal
BIOPHYSICAL JOURNAL
Volume 90, Issue 4, Pages 1241-1248Publisher
CELL PRESS
DOI: 10.1529/biophysj.105.069435
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Funding
- NIGMS NIH HHS [R01 GM065534, R01 GM070622, GM070622, GM065534] Funding Source: Medline
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The adsorption of large unilamellar vesicles composed of various combinations of phosphatidylcholine, phosphatidylethanolamine (PE), monomethyl PE, and dimethyl PE (PE-Me-2) onto a glass surface was studied using fluorescence microscopy. The average lipid geometry within the vesicles, described mathematically by the average intrinsic curvature, C-0,C-ave, was methodically altered by changing the lipid ratios to determine the effect of intrinsic curvature on the ability of vesicles to rupture and form a supported lipid bilayer. We show that the ability of vesicles to create fluid planar bilayers is dependent on C-0,C-ave and independent of the identity of the component lipids. When the C-0,C-ave was; similar to-0.1 nm(-1), the vesicles readily formed supported lipid bilayers with almost full mobility. In contrast, when the C-0,C-ave ranged from; similar to-0.2 to; similar to-0.3 nm(-1), the adsorbed vesicles remained intact upon the surface. The results indicate that the average shape of lipid molecules within a vesicle (C-0,C-ave) is essential for determining kinetically viable reactions that are responsible for global geometric changes.
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