Journal
FEBS LETTERS
Volume 580, Issue 5, Pages 1365-1370Publisher
WILEY
DOI: 10.1016/j.febslet.2006.01.058
Keywords
hnRNP A1; nucleocytoplasmic transport; transportin; NLS; NES
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Heterogeneous nuclear ribonucleoprotein (hnRNP) At regulates mRNA genesis. It shuttles between the nucleus and cytoplasm. Its shuttling signal is a 38-residue sequence M9. We studied the nuclear import and export of M9 by mutational analysis. Heterokaryon assay indicated that the 19-residue sequence SNFGPNMGGNFGGRSSGPY (M9 core) is necessary and sufficient for shuttling. Moreover, M9 core mutation revealed that in addition to the hitherto characterized N-terminal motif SNFGPMK, the C-terminal motif PY is crucial for nuclear import as well as for binding to transportin. Key residues of the motifs are conserved in the shuttling signals of hnRNP D and JKTBP. (c) 2006 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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