Journal
BIOPHYSICAL CHEMISTRY
Volume 119, Issue 3, Pages 295-302Publisher
ELSEVIER
DOI: 10.1016/j.bpc.2005.09.025
Keywords
cationic porphyrin; phenylpiperazine; intercalation; apparent affinity binding constant; photocleavage
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The binding properties of cationic porphyrin-phenylpiperazine hybrids to calf thymus (CT) DNA were investigated by using absorption, fluorescence and circular dichroism (CD) spectra, and the apparent affinity binding constants (K-app) of the porphyrins for CT DNA were determined by using a competition method with ethidium bromide (EB). Intercalation of porphyrin into CT DNA occurred when two phenylpiperazines were introduced at cis position onto the periphery of cationic porphyrin. The photocleavages of pBR322 plasmid DNA by the porphyrins were consistent with the values of K-app. With [porphyrin]/[DNA base pairs] ratio increased, the binding mode tended to be outside binding, and the cleavage abilities of the porphyrins varied. In the presence of sodium azide, a quencher of O-1(2), the cleavage of DNA by the porphyrin of intercalation was less inhibited. (C) 2005 Elsevier B.V. All rights reserved.
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