Journal
ANALYTICAL CHEMISTRY
Volume 78, Issue 3, Pages 956-958Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ac0513865
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Funding
- NHGRI NIH HHS [1R01 HG002644-01A1] Funding Source: Medline
- NIGMS NIH HHS [T32GM07616] Funding Source: Medline
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The development of microfluidic tools for high-throughput nucleic acid analysis has become a burgeoning area of research in the post-genome era. Here, we have developed a microfluidic chip to perform 72 parallel 450-pL RT-PCRs. We took advantage of Taqman hydrolysis probe chemistry to detect RNA templates as low as 34 copies. The device and method presented here may enable highly parallel single cell gene expression analysis.
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