Journal
FEMS MICROBIOLOGY LETTERS
Volume 255, Issue 2, Pages 233-239Publisher
OXFORD UNIV PRESS
DOI: 10.1111/j.1574-6968.2005.00060.x
Keywords
Staphylococcus aureus; methicillin resistance; teicoplanin resistance; MALDI-TOF mass spectrometry; peptidoglycan muropeptide analysis; pulsed-field gel electrophoresis
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To explore the discriminatory power of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) for detecting subtle differences in isogenic isolates, we tested isogenic strains of Staphylococcus aureus differing in their expression of resistance to methicillin or teicoplanin. More important changes in MALDI-TOF MS spectra were found with strains differing in methicillin than in teicoplanin resistance. In comparison, very minor or no changes were recorded in pulsed-field gel electrophoresis profiles or peptidoglycan muropeptide digest patterns of these strains, respectively. MALDI-TOF MS might be useful to detect subtle strain-specific differences in ionizable components released from bacterial surfaces and not from their peptidoglycan network.
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