Pedobacter lignilitoris sp nov., isolated from wood falls

A Gram-stain-negative, aerobic, non-motile and rod-shaped bacterial strain, designated W-WS13(T), was isolated from wood falls collected around Wando, an island in the South Sea of South Korea, and subjected to a polyphasic taxonomic study. Strain W-WS13(T) grew optimally at 30 degrees C, at pH 7.5 and in the presence of 0.5 % NaCl A neighbour-joining phylogenetic tree based on 16S rRNA gene sequences showed that strain W-WS13(T) belonged to the genus Pedobacter, clustering robustly with the type strain of Pedobacter arcticus, sharing 95.9 % sequence similarity. Strain W-WS13(T) exhibited 16S rRNA gene sequence similarity of 90.1-95.6 % to the type strains of the other species of the genus Pedobacter. Strain W-WS13(T) contained MK-7 as the predominant menaquinone and iso-C-15:0, summed feature 3 (C-16:1 omega 7c and/or C-16:1 omega 6c) and iso-C-17:0 3-OH as the major fatty acids. The major polar lipid detected in strain W-WS13T was phosphatidylethanolamine. The DNA G+C content of strain W-WS13(T) was 36.9 mol%. Phylogenetic distinctiveness and differential phenotypic properties of strain W-WS13(T) revealed that the novel strain is separated from recognized species of the genus Pedobacter. On the basis of the data presented, strain W-WS13T is considered to represent a novel species of the genus Pedobacter, for which the name Pedobacter lignilitoris sp. nov. is proposed. The type strain is W-WS13(T) (=KCTC 42500(T)=CECT 8725(T)).