Journal
FEBS LETTERS
Volume 580, Issue 5, Pages 1333-1338Publisher
WILEY
DOI: 10.1016/j.febslet.2006.01.051
Keywords
bilin reductase; chromophore; phycocyanobilin; phycoerythrobilin; phytochrome; phytochromobilin
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By co-expression of heme oxygenase and various bilin reductase(s) in a single operon in conjunction with apophytochrome using two compatible plasmids, we developed a system to produce phytochromes with various chromophores in Escherichia coli. Through the selection of different bilin reductases, apophytochromes were assembled with phytochromobilin, phycocyanobilin, and phycocrythrobilin. The blue-shifted difference spectra of truncated phytochromes were observed with a phycocyanobilin chromophore compared to a phytochromobilin chromophore. When the phycoerythrobilin biosynthetic enzymes were co-expressed, E. coli cells accumulated orange-fluorescent phytochrome. The metabolic engineering of bacteria for the production of various bilins for assembly into phytochromes will facilitate the molecular analysis of photoreceptors. (c) 2006 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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