Journal
MOLECULAR BIOSYSTEMS
Volume 2, Issue 2, Pages 122-126Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/b515923g
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We have developed a new concept involving a single-step homogeneous method for single-nucleotide polymorphism (SNP) typing. in this method, a probe containing base-discriminating fluorescent (BDF) bases is added to a sample solution. BDF base-containing DNA usually shows only a weak fluorescence, but emits a strong blue fluorescence when it recognizes a target base at a specific site in a hybridized strand. By utilizing this feature, a simple mix-and-read SNP typing assay was achieved without any tedious probe-designing or washing processes for exclusion of hybridization error or any addition of DNA-modifying enzymes. This is very different from conventional methods. We simultaneously analyzed a number of samples with ease, with a high accuracy, using our BDF assay.
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