Winogradskyella eckloniae sp nov., a marine bacterium isolated from the brown alga Ecklonia cava

A novel bacterial strain, designated EC29(T), was isolated from the brown alga Ecklonia cave collected on Jeju Island, Republic of Korea. Cells of strain EC29T were Gram-stain-negative, aerobic, rod-shaped and motile by gliding. Growth was observed at 10-30 degrees C (optimum, 20-25 degrees C), at pH 6.0-9.5 (optimum, pH 7.5) and in the presence of 1-5% (w/v) NaCl. Phylogenetic analyses based on the 16S rRNA gene sequence revealed that the strain belonged to the genus Winogradskyella. Strain EC29T exhibited the highest 16S rRNA gene sequence similarities, of 96.5-97.8%, to the type strains of Winogradskyella pulchriflava EM106(T), Winogradskyella echinorum KMM 6211(T) and Winogradskyella ulvae KMM 6390(T). Strain EC29(T) exhibited <27% DNA DNA relatedness with Winogradskyella pulchriflava EM106T and Winogradskyella echinorum KMM 6211T. The predominant fatty acids of strain EC29(T) were iso-C-15 :0, iso-C-15 :1 G, C-15 :0, iso-C-17: 0 3-OH, iso-C-15:0 3-OH and anteiso C-15 (:) (0). The DNA G + C content was 31.1 mol% and the major respiratory quinone was menaquinone-6 (MK-6). Based on a polyphasic study, strain EC29(T) is considered to represent a novel species of the genus Winogradskyella, for which the name Winogradskyella eckloniae sp. nov. is proposed. The type strain is EC29(T) (=KCTC 32172(T)=JCM 18703(T)).