Journal
INTERNATIONAL IMMUNOLOGY
Volume 18, Issue 2, Pages 389-398Publisher
OXFORD UNIV PRESS
DOI: 10.1093/intimm/dxh378
Keywords
adhesion molecules; CD11a; cytokines; inflammatory bowel disease; mesenteric lymph nodes
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Funding
- NIDDK NIH HHS [DK64023] Funding Source: Medline
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The beta(2) integrin lymphocyte function-associated antigen-1 (LFA-1; CD11a/CD18) is important for lymphocyte trafficking and activation as well as recruitment to sites of tissue inflammation. The objective of this study was to assess the role of 'T-cell-associated' LFA-1 in the pathogenesis of chronic colitis in vivo. Transfer of CD4(+)CD25(-) T cells isolated from wild-type (wt) mice into immunodeficient recipients [recombinase-activating gene-1-deficient (RAG-1(-/-))] produced moderate to severe colitis, whereas RAG-1(-/-) mice injected with CD11a-deficient (CD11a(-/-); LFA-1(-/-)) donor T cells displayed minimal macroscopic and histological evidence of colitis. Surface expression of L-selectin, alpha(4), alpha(4)beta(7) and chemokine receptor-7 were similar for wt and CD11a(-/-) donor T cells. Attenuated disease in the CD11a(-/-) -> RAG-1(-/-) animals was associated with decreased numbers of CD4(+) T cells in the mesenteric lymph nodes (MLNs), spleen and intestinal lamina propria (LP). In addition, significant reductions in T(h)1 cytokines were observed following ex vivo stimulation of mononuclear cells obtained from the MLNs and colonic LP. Interestingly, mononuclear cells obtained from the spleens of CD11a(-/-) -> RAG-1(-/-) exhibited enhanced pro-inflammatory cytokine production compared with splenocytes obtained from wt -> RAG-1(-/-) colitic mice. Taken together, our data suggest that T-cell-associated CD11a (LFA-1) expression plays a dual role in the initiation of chronic gut inflammation by facilitating naive T-cell priming/activation and expansion within MLNs and by augmenting pro-inflammatory cytokine production following secondary stimulation by antigen-presenting cells in the colonic interstitium.
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