Journal
RESEARCH IN VETERINARY SCIENCE
Volume 80, Issue 1, Pages 116-119Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.rvsc.2005.03.002
Keywords
dogs; canine distemper; canine distemper virus; urine; RT-PCR
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In a prospective study we evaluated the use of the reverse transcriptase-polymerase chain reaction (RT-PCR) in urine samples to diagnose canine distemper virus in dogs with progressive neurological disease. A fragment of the nucleoprotein gene of canine distemper virus was amplified from the urine of 22 distemper dogs. The body fluids and leukocytes of 12 asymptomatic dogs were RTPCR negative. RT-PCR of urine samples was more sensitive than serum and leukocytes and at least as sensitive as cerebrospinal fluid to screen for distemper in dogs with neurological signs and extraneural systemic signs. (c) 2005 Elsevier Ltd. All rights reserved.
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