Journal
JOURNAL OF CHEMOTHERAPY
Volume 18, Issue 1, Pages 56-65Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1179/joc.2006.18.1.56
Keywords
imatinib; endothelial cells; angiogenesis; VE-cadherin; apoptosis
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In this study the EA.hy 926 endothelial cell line-simulating endothelial cells-was treated with imatinib in order to define a possible anti-angiogenic role for imatinib. Dose and time response experiments were performed. Cell morphology was studied, while migration efficiency, intercellular permeability and VE-cadherin expression were assayed, both in the presence and in the absence of imatinib. Imatinib-nduced EA.hy 926 cell apoptosis was also examined. Results showed that imatinib reduced the endothelial cell population, changed cell monolayer morphology and reduced cell-to-cell cohesiveness. Migration efficiency was significantly decreased while intercellular permeability was 2.76-fold increased in the presence of imatinib. Indirect immunofluorescence microscopy showed nearly complete down-regulation of VE cadherin in imatinib-treated cells. Furthermore, apoptotic activity was detected in imatinib-treated cells. Altogether our results support an antiangiogenic profile for imatinib that possibly contributes to its therapeutic potential.
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