Journal
FEMS MICROBIOLOGY LETTERS
Volume 255, Issue 1, Pages 89-95Publisher
OXFORD UNIV PRESS
DOI: 10.1111/j.1574-6968.2005.00055.x
Keywords
Halobacillus dabanensis; moderately halophilic bacterium; Na+/H+ antiporter; nhaH
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A gene encoding a Na+/H+ antiporter was cloned from a chromosomal DNA of Halobacillus dabanensis strain D-8(T) by functional complementation. Its presence enabled the antiporter-deficient Escherichia coli strain KNabc to survive in the presence of 0.2 M NaCl or 5 mM LiCl. The gene was sequenced and designated as nhaH. The deduced amino-acid sequence of NhaH consists of 403 residues with a calculated molecular mass of 43 481 Da, which was 54% identical and 76% similar to the NhaG Na+/H+ antiporter of Bacillus subtilis. The hydropathy profile was characteristic of a membrane protein with 12 putative transmembrane domains. Everted membrane vesicles prepared from E. coli cells carrying nhaH exhibited Na+/H+ as well as Li+/H+ antiporter activity, which was pH-dependent with highest activities at pH 8.5-9.0 and at pH 8.5, respectively. Moreover, nhaH confers upon E. coli KNabc cells the ability to grow under alkaline conditions.
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