Journal
JOURNAL OF SEPARATION SCIENCE
Volume 29, Issue 3, Pages 399-404Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jssc.200500386
Keywords
Alexandrium tamarense extracts; capillary electrophoresis; isotachophoresis; paralytic shellfish toxins
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Baseline separation of seven paralytic shellfish toxins (PSTs), namely decarbamoylsaxitoxin (dcSTX), saxitoxin (STX), neosaxitoxin (NEO), gonyautoxin-2 (GTX-2), gonyautoxin-3 (GTX-3), gonyautoxin-1 (GTX-1), and gonyautoxin-4 (GTX-4), was achieved by using capillary ITP (CITP)/CE with UV detection. Separation parameters including duration time and voltage in CITP process, separation voltage, and pH and concentration of buffer were optimized. The developed method provided linear responses from 1.3 to 200 mu M for the PSTs. The LOD ranged from 0.1 to 0.3 mu M. PST extracts from two algal strains of Alexandrium tamarense were analyzed and the toxin concentrations in the samples were quantified with an internal standard method by using NEO as the internal standard. The algal extract of A. tamarense HK9301 contained 332 mu M GTX-2 and 224 mu M GTX-3, while the PSTs were not detected in the extract of A. tamarense CI01.
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