Flavobacterium palustre sp nov., isolated from wetland soil

A Gram-staining-negative, non-motile, yellow-coloured, rod-shaped bacterium, designated S44(T), was isolated from bankside soil of Xixi wetland, located in Zhejiang province, China. Growth of strain S44(T) was observed at 6-37 degrees C (optimum, 28 degrees C) and at pH 6.0-9.0 (optimum, 7.0). No growth occurred in the presence of >2% (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain S44(T) represented a member of the genus Flavobacterium, showing the highest sequence similarities to the sequences from Flavobacterium succinicans DSM 4002(T) (96.9%), Flavobacterium reichenbachii WB 3.2-61(T) (96.6 %) and Flavobacterium glycines NCBI 105008(T) (96.5 %). The G+C content of the genomic DNA was 33.6 mol%. The predominant cellular fatty acids were C-15:0 iso-C-15:0, anteiso-C-15:0 and summed feature 3 (comprising C-16:1 omega 7C and/or iso-C-15:0 2-OH), and the major respiratory quinone was menaquinone 6 (MK-6). The major polar lipids were phosphatidylethanolamine, two unknown aminolipids, two unknown aminophospholipids and four unknown polar lipids. On the basis of the phenotypic and genotypic data, it is proposed that the isolate S44(T) be classified as representing a novel species of the genus Flavobacterium, for which the name Flavobacterium palustre sp. nov. is proposed. The type strain is S44(T) (=CGMCC 1.12811(T)= NBRC 110389(T)).