Journal
JOURNAL OF PLANT PHYSIOLOGY
Volume 163, Issue 2, Pages 206-212Publisher
ELSEVIER GMBH
DOI: 10.1016/j.jplph.2005.01.006
Keywords
Arisaema heterophyllum agglutinin; artificial diet assay; bacterial expression; peach potato aphid (Myzus persicae); recombinant protein
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Recombinant Arisaema heterophyllum agglutinin (AHA) was expressed in Escherichia coli as N-terminal His-tagged fusions. After induction with isopropylthio-beta-D-galactoside, the recombinant AHA was purified by metal-affinity chromatography. The purified AHA protein was incorporated into artificial diet at 0.1% (w/v) concentration in insect bioassay trial and the result showed that artificial diet containing AHA could significantly inhibit the growth of the third-instar nymphs of peach potato aphid (Myzus persicae). This study suggested that AHA could be an effective candidate for the control of peach potato aphid, one of the most serious sap-sucking insect pests causing significant yield loss of crops. (C) 2005 Elsevier GmbH. All rights reserved.
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