Journal
JOURNAL OF BIOENERGETICS AND BIOMEMBRANES
Volume 38, Issue 1, Pages 75-82Publisher
SPRINGER/PLENUM PUBLISHERS
DOI: 10.1007/s10863-006-9008-3
Keywords
lysosome; arachidonic acid; permeability; potassium ion; proton; osmotic sensitivity
Categories
Ask authors/readers for more resources
In this study, we investigated the effects of arachidonic acid, a PLA(2)-produced lipid metabolite, on the lysosomal permeability, osmotic sensitivity and stability. Through the measurements of lysosomal beta-hexosaminidase free activity, membrane potential, intralysosomal pH, and lysosomal latency loss in hypotonic sucrose medium, we established that arachidonic acid could increase the lysosomal permeability to both potassium ions and protons, and enhance the lysosomal osmotic sensitivity. As a result, the fatty-acid-promoted entry of potassium ions into the lysosomes via K+/H+ exchange, which could produce osmotic imbalance across their membranes and osmotically destabilize the lysosomes. In addition, the enhancement of lysosomal osmotic sensitivity caused the lysosomes to become more liable to destabilization in osmotic shock. The results suggest that arachidonic acid may play a role in the lysosomal destabilization.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available