Journal
JOURNAL OF PROTEOME RESEARCH
Volume 5, Issue 2, Pages 248-253Publisher
AMER CHEMICAL SOC
DOI: 10.1021/pr050269n
Keywords
histone H2A; Fourier transform mass spectrometry (FTMS); Top Down; post-translational modification (PTM); SILAC; cell cycle; ubiquitination; electron capture dissociation (ECD); histone code; chromatin
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Funding
- NIGMS NIH HHS [GM 067193-03, 5T32 GM 07283] Funding Source: Medline
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Top Down analysis revealed that at least fourteen genes encoding histone H2A are coexpressed in HeLa cells. Characterization of these species' revealed that all except H2A.Z and H2A.F/Z were alpha-N-acetylated, H2A.O and H2A.C,D,I,N,P were the most abundant, and those exceeding similar to 10% abundance lacked post-translational modifications. This unequivocal identification of H2A forms illustrates the advantages of Top Down Mass Spectrometry and provides a global perspective of H2A regulation through the cell cycle.
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