Journal
JOURNAL OF NEUROSCIENCE
Volume 26, Issue 5, Pages 1531-1538Publisher
SOC NEUROSCIENCE
DOI: 10.1523/JNEUROSCI.4636-05.2006
Keywords
ROR alpha; Purkinje cells; dendritic regression; differentiation; lentiviral vector; organotypic culture
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Dendritic differentiation involves both regressive and growth events. The mechanisms controlling the regressive events are poorly understood. This study is aimed at determining the role of the nuclear receptor retinoid-related orphan receptor alpha (ROR alpha) in Purkinje cell (PC) dendritic differentiation in organotypic cultures. As observed in vivo, in these cultures, fusiform PCs with embryonic bipolar shape undergo regression before the outgrowth of the ultimate dendritic tree. We show that lentiviral-mediated hROR alpha 1 overexpression in fusiform PCs leads to a cell-autonomous accelerated progression of dendritic differentiation. In addition, ROR alpha is necessary for the PC regressive events: whereas staggerer ROR alpha-deficient PCs remain in the embryonic fusiform stage, replacement of hROR alpha 1 restores normal dendritogenesis. These results demonstrate that ROR alpha expression in fusiform PCs is crucial for the dendritic regression and progression of the following step of extension of dendritic processes. However, it does not seem to participate to the last stage of dendritic growth. This study identifies ROR alpha as a nuclear receptor crucial for the control of dendritic remodeling during development.
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