Undibacterium aquatile sp nov., isolated from a waterfall

A Gram-stain-negative, aerobic, motile and rod-shaped strain, THG-DN7.3(T), was isolated from a waterfall. Strain THG-DN7.3(T) grew well at 18-28 degrees C and at pH 6.0-7.5 on Reasoner's 2A agar. Based on 16S rRNA gene sequence comparisons, strain THG-DN7.3(T) was most closely related to Undibacterium jejuense JS4-4(T) (97.3 % 16S rRNA gene sequence similarity) and Undibacterium seohonense SHS5-24(T) (96.5 %). The G+C content of the genomic DNA was 57.4 mol%. The mean DNA-DNA relatedness of strain THG-DN7.3(T) with U. jejuense KACC 12607(T) was 40 +/- 1 % (reciprocal 50 +/- 2.1 %). The major cellular fatty acids of strain THG-DN7.3(T) were summed feature 3 (C-16 : 1 omega 7c and/or C-16 : 1 omega 6c) (47.4 %), C-16 : 0 (30.4 %), summed feature 8 (C-18 : 1 omega 6c and/or C-18 : 1 omega 7c) (6.8 %) and C-12 : 0 (6.2 %). The predominant isoprenoid quinone was ubiquinone-8. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. The results of the DNA-DNA hybridization and genotypic analysis, in combination with chemotaxonomic and physiological data, demonstrated that strain THG-DN7.3(T) represents a novel species of the genus Undibacterium, for which the name Undibacterium aquatile sp. nov. is proposed. The type strain is THG-DN7.3(T) (=KCTC 42243(T) =CCTCC AB 2015119(T)).