Journal
ANNALS OF THE RHEUMATIC DISEASES
Volume 72, Issue 10, Pages 1680-1686Publisher
BMJ PUBLISHING GROUP
DOI: 10.1136/annrheumdis-2012-202407
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- Wyeth Pharmaceuticals
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Background and objectives Therapeutic monoclonal antibodies are effective drugs for many different diseases. However, the formation of anti-drug antibodies (ADA) against a biological can result in reduced clinical response in some patients. Measurement of ADA in the presence of (high) drug levels is difficult due to drug interference in most assays, including the commonly used antigen binding test (ABT). Methods We recently published a novel method which enables the measurement of complexed antibodies against adalimumab (an anti-TNF antibody) in the presence of drug. Here we use this pH-shift-anti-idiotype ABT (PIA) to measure anti-adalimumab antibodies (AAA) in 99 rheumatoid arthritis (RA) patients treated for up to 3years with adalimumab. Results 53 out of 99 RA patients produced AAA. In 50 of these PIA positive patients, AAA could be detected within the first 28weeks of treatment. Patients in which AAA could be detected in the PIA after 28weeks of treatment were more prone to declining adalimumab levels (<5 mu g/ml) (p<0.01) and high AAA levels which could be detected in the ABT (p<0.05) at later time points. We observed transient AAA formation in 17/53 patients. Conclusions Results show that AAA develop early in treatment. However, levels that completely neutralise the drug may be reached much later in treatment. Furthermore, the patients positive for PIA at 28weeks have an increased chance to develop clinical non-response due to immunogenicity. In some of the patients, AAA formation is transient.
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