Journal
ANALYTICAL LETTERS
Volume 39, Issue 3, Pages 483-494Publisher
TAYLOR & FRANCIS INC
DOI: 10.1080/00032710500535956
Keywords
biosensor; horseradish peroxidase; nafion; Au nanoparticle; polythionine; gelatin
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A new biosensor for the amperometric detection of hydrogen peroxide was developed by means of immobilized horseradish peroxidase (HRP) on a platinum disk based on gold nanoparticles, nafion, polythionine (PTn), and gelatin as matrixes. The mediator (PTn) was embedded in nafion film effectively without leaching even after long periods of operation, the immobilization of the enzyme comes from the cooperative binding by the Au nanoparticles and gelatin. The fabrication procedure of the biosensor was characterized by using cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The electrochemical characteristics of the enzyme electrode with respect to the effect of pH, temperature, and the operational and storage stabilities were studied. The test demonstrated that the biosensors show high stability, fast response (< 20 s ). The analytical results by this approach were in satisfactory agreement with those by conventional methods of titration.
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