Halobacillus sediminis sp nov., a moderately halophilic bacterium isolated from a solar saltern sediment

A Gram-staining-positive, moderately halophilic bacterium, designated strain NGS-2(T), was isolated from sediment of a solar saltern pond located in Shinan, Korea. Strain NGS-2(T) was a strictly aerobic, non-motile rod that grew at pH 5.0-10.0 (optimum, pH 8.0), at 10-30 degrees C (optimum, 28 degrees C) and in the presence of 1-20 % (w/v) NaCl (optimum, 10 % NaCl). Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain NGS-2(T) belonged to the genus Halobacillus, with sequence similarity of 98.4-95.8 % to existing type strains, showing the highest sequence similarity to Halobacillus dabanensis D-8(T) (98.4 %), H. litoralis SL-4(T) (98.4 %), H. trueperi SL-5(T) (98.2 %), H. faecis IGA7-4(T) (98.2 %), H. profundi IS-Hb4(T) (98.1 %) and H. mangrovi MS10(T) (98.0 %). The major polar lipids were phosphatidylglycerol, phosphatidylethanolamine, phosphatidyl-N-methylethanolamine and an unknown glycolipid. The cell-wall peptidoglycan was based on L-Orn-D-Asp, the predominant isoprenoid quinone was menaquinone 7 (MK-7).and the major fatty acids were anteiso-C-15 : 0 and anteiso-C-17 : 0. The DNA G +C content of the novel isolate was 45.0 mol%. Levels of DNA DNA relatedness between strain NGS-2(T) and the type strains of 12 other species of the genus ranged from 32 to 3 %. On the basis of the polyphasic analysis conducted in this study, strain NGS-2(T) represents a novel species of the genus Halobacillus, for which the name Halobacillus sediminis sp. nov. is proposed. The type strain is NGS-2(T) (=KACC 18263(T)=NBRC 110639(T)).