Journal
JOURNAL OF MOLECULAR BIOLOGY
Volume 355, Issue 5, Pages 1048-1065Publisher
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.jmb.2005.11.021
Keywords
transferrin receptor-transferrin complex; specimen preparation; angular reconstitution; random conical tilt; cryo-negative staining
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Funding
- NIGMS NIH HHS [GM62580] Funding Source: Medline
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The outcome of three-dimensional (3D) reconstructions in single particle electron microscopy (EM) depends on a number of parameters. We have used the well-characterized structure of the transferrin (Tf)-transferrin receptor (TfR) complex to study how specimen preparation techniques influence the outcome of single particle EM reconstructions. The Tf-TfR complex is small (290 kDa) and of low symmetry (2-fold). Angular reconstitution from images of vitrified specimens does not reliably converge on the correct structure. Random conical tilt reconstructions from negatively stained specimens are reliable, but show variable degrees of artifacts depending on the negative staining protocol. Alignment of class averages from vitrified specimens to a 3D negative stain reference model using FREALIGN largely eliminated artifacts in the resulting 3D maps, but not completely. Our results stress the need for critical evaluation of structures determined by single particle EM. (c) 2005 Elsevier Ltd. All rights reserved.
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