Journal
EXPERIMENTAL CELL RESEARCH
Volume 312, Issue 4, Pages 423-433Publisher
ELSEVIER INC
DOI: 10.1016/j.yexcr.2005.11.005
Keywords
3D collagen gel; tissue bioreactor; matrix tension; cytochalasin D; TGF beta 1; mechanical loading; collagen remodeling
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The absence of a controllable in vitro model of soft tissue remodeling is a major impediment, limiting our understanding of collagen pathologies, tissue repair and engineering. Using 3D fibroblast-collagen lattice model, we have quantified changes in matrix tension and material properties following remodeling by blockade of cell-generated tension with cytochalasin D. This demonstrated a time-dependent shortening of the collagen network, progressively stabilized into a built-in tension within the matrix. This was differentlially enhanced by TGFBI and mechanical loading to give subtle control of the new, remodeled matrix material properties. Through this model, we have been able to identify the 'tension remodeling' process, by which cells control material properties in response to environmental factors. (C) 2005 Elsevier Inc. All rights reserved.
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