Journal
ANALYTICAL BIOCHEMISTRY
Volume 349, Issue 2, Pages 176-180Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ab.2005.03.007
Keywords
caffeine; theophylline; spectrophotometry; sodium metaperiodate; MBTH; pharmaceuticals
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A novel-coupling reagent is used for the simple and sensitive spectrophotometric determination of caffeine (CF) and theophylline (TP) in pure or pharmaceutical formulations. The method is based on the oxidation of CF and TP with sodium metaperiodate in the presence of acetic acid, followed by coupling with 3-methyl 2-benzo thiazolinone hydrazone hydrochloride (MBTH), to produce a blue-colored product with lambda(max) of 630 nm. The method is reproducible and has been applied for the determination of CF and TP in the tablets. The results are comparable to those obtained with the British pharmacopoeia (BP) method. Common excipients used as additives in pharmaceutical preparations do not interfere in the proposed method. (c) 2006 Published by Elsevier Inc.
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