Journal
JOURNAL OF BIOTECHNOLOGY
Volume 121, Issue 4, Pages 563-569Publisher
ELSEVIER
DOI: 10.1016/j.jbiotec.2005.08.017
Keywords
cyanobacterium; phycocyanin; phycoerythrin; fluorescent probe; Spirulina platensis
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Phycocyanin (PC) prepared from a cyanobacterium Spirulina platensis by the DEAE-DE52 cellulose column chromatography that was developed by gradient elution of 50-250 mM phosphate buffer (pH 7.0) was stabilized by its subunits cross-linked covalently with formaldehyde. The single blue band that the chemically stabilized PC showed in sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) demonstrated that the stabilized PC still maintained its trimeric aggregate form even after its incubation at 60 degrees C for 3 h and at 100 degrees C for 10 min in the denatured buffer containing 5% (w/v) SDS. Moreover, the stabilized PC exhibited similar spectroscopic properties of absorption and fluorescence to those of the native PC, and showed adequate energy coupling with R-phycoerythrin (R-PE) after it was conjugated with R-PE via glutaraldehyde. (c) 2005 Elsevier B.V. All rights reserved.
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