Journal
JOURNAL OF BIOLOGICAL CHEMISTRY
Volume 281, Issue 8, Pages 5050-5057Publisher
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
DOI: 10.1074/jbc.M508550200
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Funding
- NCI NIH HHS [5P30CA023074] Funding Source: Medline
- NHLBI NIH HHS [R01HL56595] Funding Source: Medline
- NIGMS NIH HHS [R0GM42474] Funding Source: Medline
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We developed a ligand-mimetic antibody Fab fragment specific for Drosophila alpha PS2 beta PS integrins to probe the ligand binding affinities of these invertebrate receptors. TWOW-1 was constructed by inserting a fragment of the extracellular matrix protein Tiggrin into the H-CDR3 of the alpha v beta 3 ligand-mimetic antibody WOW-1. The specificity of alpha PS2 beta PS binding to TWOW-1 was demonstrated by numerous tests used for other integrin-ligand interactions. Binding was decreased in the presence of EDTA or RGD peptides and by mutation of the TWOW-1 RGD sequence or the beta PS metal ion-dependent adhesion site ( MIDAS) motif. TWOW-1 binding was increased by mutations in the alpha PS2 membrane-proximal cytoplasmic GFFNR sequence or by exposure to Mn2+. Although Mn2+ is sometimes assumed to promote maximal integrin activity, TWOW-1 binding in Mn2+ could be increased further by the beta PS2 GFFNR -> GFANA mutation. A mutation in the beta PS I domain ( beta PS-b58; V409D) greatly increased ligand binding affinity, explaining the increased cell spreading mediated by alpha PS2 beta PS-b58. Further mutagenesis of this residue suggested that Val-409 normally stabilizes the closed head conformation. Mutations that potentially reduce interaction of the integrin beta subunit plexin-semaphorin-integrin ( PSI) and stalk domains have been shown to have activating properties. We found that complete deletion of the beta PS PSI domain enhanced TWOW-1 binding. Moreover the PSI domain is dispensable for at least some other integrin functions because beta PS-Delta PSI displayed an enhanced ability to mediate cell spreading. These studies establish a means to evaluate mechanisms and consequences of integrin affinity modulation in a tractable model genetic system.
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