Journal
SENSORS AND ACTUATORS B-CHEMICAL
Volume 113, Issue 2, Pages 956-962Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2005.04.002
Keywords
immunosensor; magnetic bio-nanoparticles; sandwich immunoassay; IgG
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An amperometric immunosensor was developed based on immobilizing antibody on the surface of modified CdFe2O4 magnetic nanoparticles. The goat anti-human IgG antibody (anti-IgG) was first covalently immobilized to core-shell (CdFe2O4-SiO2) magnetic nanoparticles, which were surface-modified with amino groups on the surface. The resulting bio-magnetic nanoparticles were attached to the surface of carbon paste electrode (CPE) with the help of a permanent magnet. A sandwich immunoassay was performed on the bio-magnetic nanoparticles monolayer supported by CPE. The assay comprised first loading of IgG on the bio-magnetic nanoparticles monolayer, then blocking in BSA solution, followed by incubation in anti-IgG-HRP conjugate, and finally the amperometric detection with hydroquinone as a mediator. The linear range for IgG assay was 0.51-30.17 mu g ml(-1) and the detection limit was 0.18 mu g ml(-1). The simple manipulations of construction and low cost were main advantages of proposed immumosensing method. (c) 2005 Elsevier B.V. All rights reserved.
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