Interleukin-12 induces a Th1-like response to Burkholderia mallei and limited protection in BALB/c mice

We evaluated the effect of interleukin (IL)-12 on the immune response to Burkholderia mallei in BALB/c mice. Mice were vaccinated with non-viable B. mallei cells with or without IL-12. There was a seven- to nine-fold increase in IgG2a levels, and a significant increase in the proliferative response and interferon (IFN)-gamma production by splenocytes from mice that received B. mallei and IL-12. We saw an increase in survivors in the groups of mice that received B. mallei and IL-12 when challenged, compared to mice that received only B. mallei or IL-12. The results Suggest that IL-12 can enhance the Th1-like immune response to B. mallei and mediate limited protection from a lethal challenge. (c) 2005 Elsevier Ltd. All rights reserved.