Journal
JOURNAL OF HAZARDOUS MATERIALS
Volume 129, Issue 1-3, Pages 216-222Publisher
ELSEVIER
DOI: 10.1016/j.jhazmat.2005.08.040
Keywords
phenol; biodegradation; Bacillus brevis; batch reactor; kinetics
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A strain of Bacillus brevis has been isolated and identified based on biochemical results. Phenol biodegradation in a batch reactor was studied using the pure culture of B. brevis. The isolated strain was optimized for various environmental conditions and the biodegradation of phenol was maximum at pH 8.0, 5% (v/v) of inoculum size and without any co-substrate. The kinetics of biodegradation according to Haldane's equation mu = mu(max)S/(K-s + S+ (S-2/K-i)) adequately describes cell growth with kinetic constants in the ranges mu(max) = 0.026-0.078 h(-1), K-s = 2.2-29.31 mg/l, K-i = 868.0-2434.7 mg/l. These values are specific for this organism and we have compared with literature for pure or mixed cultures degrading phenol. (c) 2005 Elsevier B.V. All rights reserved.
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