Journal
BRAZILIAN JOURNAL OF MEDICAL AND BIOLOGICAL RESEARCH
Volume 39, Issue 3, Pages 405-410Publisher
ASSOC BRAS DIVULG CIENTIFICA
DOI: 10.1590/S0100-879X2006000300011
Keywords
methylmercury; retina; Hoplias malabaricus; amacrine cells; displaced amacrine cells; ON-bipolar cells
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Funding
- Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [02/12733-8] Funding Source: FAPESP
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To quantify the effects of methylmercury (MeHg) on amacrine and on ON-bipolar cells in the retina, experiments were performed in MeHg-exposed groups of adult trahiras (Hoplias malabaricus) at two dose levels (2 and 6 mu g/g, ip). The retinas of test and control groups were processed by mouse anti-parvalbumin and rabbit anti-(alpha)protein kinase C(alphaPKC) immunocytochemistry. Morphology and soma location in the inner nuclear layer were used to identify immunoreactive parvalbumin (PV-IR) and alphaPKC (alphaPKC-IR) in wholemount preparations. Cell density, topography and isodensity maps were estimated using confocal images. PV-IR was detected in amacrine cells in the inner nuclear layer and in displaced amacrine cells from the ganglion cell layer, and alphaPKC-IR was detected in ON-bipolar cells. The MeHg-treated group (6 mu g/g) showed significant reduction of the ON-bipolar alphaPKC-IR cell density (mean density = 1306 +/- 393 cells/mm(2)) Compared to control (1886 +/- 892 cells/mm(2); P < 0.001). The mean densities found for amacrine PV-IR cells in MeHg-treated retinas were 1040 +/- 56 cells/mm(2) (2 mu g/g) and 845 +/- 82 cells/mm(2) (6 mu g/g), also lower than control (1312 +/- 31 cells/mm(2); P < 0.05), differently from the data observed in displaced PV-IR amacrine cells. These results show that MeHg changed the PV-IR amacrine cell density in a dose-dependent way, and reduced the density of alphaPKC-IR bipolar cells at the dose of 6 mu g/g. Further studies are needed to identify the physiological impact of these findings on visual function.
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