Journal
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
Volume 143, Issue 3, Pages 294-301Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.cbpb.2005.11.017
Keywords
central pattern generator; STG; signal transduction; cAMP; neuron; invertebrate; neuromodulation; G protein coupled receptor
Categories
Funding
- NINDS NIH HHS [NS38770, R01 NS038770-05, R01 NS038770] Funding Source: Medline
Ask authors/readers for more resources
Dopamine (DA) differentially modulates identified neurons in the crustacean stomatogastric nervous system (STNS). While the electrophysiological actions of DA have been well characterized, little is known about the dopaminergic transduction cascades operating in this system. As a first step toward illuminating the molecular underpinnings of dopaminergic signal transduction in the crustacean STNS, we have cloned and characterized two type-one DA receptors (DARs) from the spiny lobster (Panulirus interruptus): D-1 alpha Pan and D-1 beta Pan. We found that the structure and function of these arthropod DARs are well conserved across species. Using a heterologous expression system, we determined that DA, but not serotonin, octopamine, tyramine or histamine activates these receptors. When stably expressed in HEK cells, the D-1 alpha Pan receptor couples with Gs, and DA elicits an increase in [CAMP]. The D-1 beta Pan receptor responds to DA with a net increase in [CAMP] that is mediated by Gs and Gz. (C) 2005 Elsevier Inc. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available