Journal
JOURNAL OF PROTEOME RESEARCH
Volume 5, Issue 3, Pages 502-511Publisher
AMER CHEMICAL SOC
DOI: 10.1021/pr050430u
Keywords
bromodomain; multiprotein complexes; affinity chromatography; high pressure liquid chromatography; mass spectral analysis; transcription factor; TATA binding protein-associated factors; chromatin assembly and disassembly; cyclin A
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Funding
- NCI NIH HHS [R29 CA075107-05, CA75107, R03 CA128006-02, CA102889, R01 CA084193, R29 CA075107-04, R03 CA128006, CA84193, R03 CA102889-02, R03 CA128006-01, R03 CA102889, R03 CA102889-01A1, R29 CA075107, R29 CA075107-03] Funding Source: Medline
- NCRR NIH HHS [P41 RR010888-05S10020, P41 RR010888-040020, P41 RR010888-090116, P41 RR010888-107084, P41 RR010888-118005, P41 RR010888-030019, P41 RR010888-050020, P41 RR010888-136129, P41 RR010888, P41 RR010888-125184, P41-RR10888, S10-RR15942, P41 RR010888-080116] Funding Source: Medline
- NHLBI NIH HHS [N01-HV-28178, N01 HV028178, N01HV28178] Funding Source: Medline
- NIGMS NIH HHS [P41 GM104603] Funding Source: Medline
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We use affinity purification of the double bromodomain protein Brd2 to isolate a multicomponent nuclear complex from cultured cells, and apply mass spectrometry/proteornics methods to identify the participants. We then confirm by immunoblot several transcription co-activators and co-repressors, proteins of the Swi/Snf chromatin remodeling complex, which regulate transcription control of cyclin A. This multiprotein complex is likely to contribute to cell cycle control and play a role in proliferation and cancer.
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