Journal
JOURNAL OF BIOCHEMISTRY
Volume 139, Issue 3, Pages 323-327Publisher
OXFORD UNIV PRESS
DOI: 10.1093/jb/mvj070
Keywords
evanescent; flow cytometry; glycan profiling; lectin; Lec mutant; microarray
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We recently developed a novel system for lectin microarray based on the evanescent-field fluorescence-detection principle, by which even weak lectin-oligosaccharide interactions are detectable without a washing procedure. For its practical application, cell glycan analysis was performed for Chinese hamster ovary (CHO) cells and their glycan profile was compared with those of their glycosylation-defective Lee mutants. Each of the cell surface extracts gave a significantly different profile from that of the parental CHO cells in a manner reflecting denoted biosynthetic features. Hence, the developed lectin microarray system is considered to be fully applicable for differential glycan profiling of crude samples.
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