Journal
FREE RADICAL RESEARCH
Volume 40, Issue 3, Pages 251-261Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/10715760500509165
Keywords
rat cardiomyocytes; polyunsaturated fatty acids; cell physiology; free radicals; xanthine/xanthine oxidase; 13-hydroperoxyoctadecatrienoic acid
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We investigated the influence of PUFA in phospholipids (PL) on the functional characteristics of cultured cardiomyocytes (CM) in basal conditions and during free radical (FR) stress provoked either by the xanthine/xanthine oxidase (X/XO) system or by a (9Z, 11 E, 13 (S), 15Z)-13-hydroperoxyoctadecatrienoic acid (13-HpOTrE). CM were grown in media containing either n-3 (eicosapentaenoic acid, EPA, and docosahexaenoic acid, DHA) or n-6 (arachidonic acid, AA). These two groups of CM displayed different PUFA n-6/n-3 ratio in PL. However, their basal electromechanical characteristics were similar. The X/XO system drastically altered CM functions, without difference between the two groups of CM. 13-HpOTrE caused a moderate and reversible depression in action potential parameters, which was dependent upon the PL PUFA, since the n-3-enriched CM exhibited an earlier functional depression but faster recovery. Thus, the peroxidative damage of CM depended on a cross relationship between FR species and the PL PUFA composition.
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