Journal
STEM CELLS
Volume 24, Issue 3, Pages 763-771Publisher
WILEY
DOI: 10.1634/stemcells.2005-0137
Keywords
fluorescence-activated cell sorting; embryonic stem cell; Sox1; teratoma; transplantation; dopaminergic neuron
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The differentiation of dopaminergic (DA) neurons from mouse embryonic stem cells (ESCs) can be efficiently induced, making these neurons a potential source for transplantation as a treatment for Parkinson's disease, a condition characterized by the gradual loss of midbrain DA neurons. One of the major persistent obstacles to the successful implementation of therapeutic ESC transplantation is the propensity of ESC-derived grafts to form tumors in vivo. To address this problem, we used fluorescence-activated cell sorting to purify mouse ESC-derived neural pre-cursors expressing the neural precursor marker Sox-1. ESC-derived, Sox1(+) cells began to express neuronal cell markers and differentiated into DA neurons upon transplantation into mouse brains but did not generate tumors in this site. In contrast, Sox1(-) cells that expressed ESC markers frequently formed tumors in vivo. These results indicate that Sox1-based cell sorting of neural precursors prevents graft-derived tumor formation after transplantation, providing a promising strategy for cell transplantation therapy of neurodegenerative disorders.
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