Journal
JOURNAL OF VIROLOGICAL METHODS
Volume 132, Issue 1-2, Pages 25-31Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jviromet.2005.08.018
Keywords
real-time PCR; lamivudine; hepatitis B virus
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A rapid and accurate minor groove binder (MGB) real-time PCR is described for detection lamivudine resistance mutations in hepatitis B virus. The real-time PCR was compared with direct Sanger sequencing in 53 clinical patients samples, the results of the real-time PCR correlate with the nucleotide sequence and the assay has the advantage of detecting a mixture of quasispecies with higher sensitivity than sequencing. As a simple, easy, rapid, accurate and high throughout method, MGB real-time PCR assay should be useful for detecting lamivudine resistance variants during lamivudine therapy in clinical settings. (c) 2005 Elsevier B.V. All rights reserved.
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