Journal
CYTOMETRY PART A
Volume 69A, Issue 3, Pages 189-191Publisher
WILEY
DOI: 10.1002/cyto.a.20232
Keywords
human macrophages; atherosclerosis; E-LDL; Ox-LDL; rafts
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Atherosclerosis is characterized by the generation of lipid-loaded macrophage-derived foam cells. To study the effect of different types of atherogenic lipoproteins, human macrophages were loaded with enzymatically degraded low density lipoprotein (E-LDL) or oxidized LDL (Ox-LDL). Cellular cholesterol content was increased by E-LDL, whereas Ox-LDL increased the ceramide content. Cell surface expression analysis by flow cytometry and confocal microscopy revealed that Ox-LDL increased ceramide and lactosylceramide expression compared to E-LDL loading and induced ceramide rafts, whereas loading with E-LDL induced cholesterol-rich microdomains. Formation of different rafts may have consequences for raft associated signaling hi cholesterol homeostasis and apoptosis in human macrophages. (c) 2006 Internatiorial Society for Analytical Cytology.
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