Journal
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
Volume 70, Issue 3, Pages 706-711Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1271/bbb.70.706
Keywords
prostaglandin; paclitaxel; PC12 cell; nerve growth factor; microtubule stabilization
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Two prostaglandins, PGA(2) and PGB(2), were isolated from the Okinawan zoanthid, Palythoa kochii, during a search for paclitaxel-like neurite-degenerating compounds from natural sources using a cell-based assay method. In the presence of PGA(2) at 30 mu M, the neuronal processes induced in PC12 cells by the nerve growth factor (NGF) degenerated over 24 h, whereas PGB(2) had no effect on the neuronal processes of PC12 cells. This activity of PGA, was similar to that of the microtubule-stabilizing agents, paclitaxel (Taxol (R)) and epothilone A, unlike the microtubule-depolymerizing agent, colchicine, which brought about quick neurite degeneration within 3h. PGA(2) stimulated tubulin polymerization, although less potently than paclitaxel. An examination of structure-activity relationships across several PGs suggests that the cyclopentenone ring structure and the orientation of its dipolar moment played an important role in the paclitaxel-like neurite-degenerating activity. These results suggest that the cyclopentenone-type PGs can interact with microtubules to inhibit their function like paclitaxel.
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