Journal
BLOOD CELLS MOLECULES AND DISEASES
Volume 36, Issue 2, Pages 191-193Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bcmd.2005.12.033
Keywords
fibroblasts; collagen secretion; extracellular matrix; fibrin matrix
Categories
Ask authors/readers for more resources
The reconstruction of extracellular matrix (ECM) by confocal reflection microscopy is a physical approach for monitoring the ECM structure, cell-matrix interactions and changes of E;CM structure over time. We here show that confocal reflection imaging is useful in reconstructing the fibrillar architecture of 3D fibrin lattices and fibroblasts embedded therein at high resolution Lip to 250 nm. Together with confocal fluorescence microscopy of collagen deposited by fibroblasts, this technique allows the monitoring of fibrin remodeling by stromal cells. In conclusion, confocal reflection microscopy is a valuable technique for real-time monitoring of the remodeling of fibrin matrices, Such as during thrombus formation, wound healing, and tumor formation. (c) 2006 Elsevier Inc. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available