Journal
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
Volume 131, Issue 1-3, Pages 795-807Publisher
HUMANA PRESS INC
DOI: 10.1385/ABAB:131:1:795
Keywords
cane juice-based medium; L-lactic acid production; optical purity; Saccharomyces cerevisiae
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We developed a metabolically engineered Saccharomyces cerevisiae, which produces optically pure L-lactic acid efficiently using cane juice-based medium. In this recombinant, the coding region of pyruvate decarboxylase (PDC)1 was completely deleted, and six copies of the bovine L-lactate dehydrogenase (L-LDH) genes were introduced on the genome under the control of the PDC1 promoter. To confirm optically pure lactate production in low-cost medium, cane juice-based medium was used in fermentation with neutralizing conditions. L-lactate production reached 122 g/L, with 61% of sugar being transformed into L-lactate finally. The optical purity of this L-lactate, that affects the physical characteristics of poly-L-lactic acid, was extremely high, 99.9% or over.
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