3.8 Article

Group-specific primers for amplifying DNA sequences that identify Amphipoda, Cephalopoda, Echinodermata, Gastropoda, Isopoda, Ostracoda and Thoracica

Journal

MOLECULAR ECOLOGY NOTES
Volume 6, Issue 1, Pages 268-271

Publisher

WILEY
DOI: 10.1111/j.1471-8286.2005.01172.x

Keywords

diet; diversity; identification; mixed; taxonomy

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We describe seven group-specific primer pairs that amplify small sections of ribosomal RNA genes suitable for identification of animal groups of major importance as prey items in marine ecosystems. These primer sets allow the isolation of DNA from the target animal groups from mixed pools of DNA, where DNA-based identification using universal primers is unlikely to succeed. The primers are designed for identifying prey in animal diets, but could be used in any situation where these animal groups are to be identified by their DNA.

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