Journal
JOURNAL OF NEUROPHYSIOLOGY
Volume 95, Issue 3, Pages 1949-1956Publisher
AMER PHYSIOLOGICAL SOC
DOI: 10.1152/jn.00959.2005
Keywords
-
Categories
Funding
- NINDS NIH HHS [NS-47508] Funding Source: Medline
Ask authors/readers for more resources
The precise role of the high concentration of ionic zinc found in the synaptic vesicles of certain glutamatergic terminals is unknown. Fluorescent probes with their ability to detect ions at low concentrations provide a powerful approach to monitoring cellular Zn2+ levels. In the last few years, a number of fluorescent probes ( indicators) have been synthesized that can be used to visualize Zn2+ in live cells. The interpretation of data gathered using such probes depends crucially on the location of the probe. Using acutely prepared hippocampal slices, we provide evidence that the Zn2+ probes, ZnAF-2 and ZP4, are membrane permeant and are able to pass into synaptic vesicles. In addition, we show that changes in fluorescence of the Zn2+ probes can be used to monitor presynaptic activity; however, these changes are inconsistent with Zn2+ release.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available