Journal
BIOMATERIALS
Volume 27, Issue 8, Pages 1341-1345Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2005.09.007
Keywords
hydrogels; protein adsorption; lysozyme; contact lens
Ask authors/readers for more resources
Lysozyme interaction with an acrylic-based hydrogel, poly(2-hydroxyethyl inethacrylate) co-methacrylic acid (P(HEMA-MAA)), was investigated using a combination of quartz crystal microbalance with dissipation (QCM-D), surface plasmon resonance (SPR) and dual polarisation interferometry (DPI). This combination of techniques demonstrated that lysozyme initially absorbed into the hydrogel matrix and displaced water from the hydrogel while subsequent lysozyme additions were adsorbed onto the surface of the hydrogel material. QCM-D, being sensitive to bound water, showed an overall decrease in mass and stiffening of the layer after lysozyme addition. SPR, a water insensitive technique, showed a net mass increase after addition of lysozyme and buffer rinses. DPI showed that the first exposure of lysozyme to P(HEMA-MAA) was consistent with lysozyme absorption while Subsequent lysozyme exposures were consistent with lysozyme adsorption. (c) 2005 Elsevier Ltd. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available