Journal
CIENCIA E AGROTECNOLOGIA
Volume 30, Issue 2, Pages 302-307Publisher
UNIV FEDERAL LAVRAS-UFLA
DOI: 10.1590/S1413-70542006000200016
Keywords
Glutathione S-transferase; extraction; bovine liver; enzyme activity
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Considering the detoxication functions of Glutathione S-transferase (GST) enzyme, that is important against oxidative stress, cancer and others degenerative diseases, this study aimed to evaluate the stability and activity of Glutathione S-transferase extracted from bovine liver, which is commercially available at low cost. The extraction was done in four steps (homogenization/centrifugation, passage through column containing diethylaminoethyl-cellulose (DEAE), precipitation with ammonium sulfate and passing through column of carboxy-methyl-cellulose (CMC). The extract thus obtained showed activity with 1 chloro 2, 4 dinitrobenzene, in the presence of reduced glutation. The specific activity of the final extract was 5 times greater than the crude centrifuged extract, and was stable for 70 days when stored at 5 degrees C.
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