Journal
INFECTION AND IMMUNITY
Volume 74, Issue 3, Pages 1973-1976Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/IAI.74.3.1973-1976.2006
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A mutant of Brucella suis bearing a Tn5 insertion in norD, the last gene of the operon norEFCBQD, encoding nitric oxide reductase, was unable to survive under anaerobic denitrifying conditions. The norD strain exhibited attenuated multiplication within nitric oxide-producing marine macrophages and rapid elimination in mice, hence demonstrating that norD is essential for Brucella virulence.
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