Laser scanning cytometry in human brain slices

Background: The Laser Scanning Cytometry (LSC) offers quantitative fluorescence analysis of cell suspensions and tissue sections. Methods: We adapted this technique to immunohistochemical labelled human brain slices. Results: We were able to identify neurons according to their labelling and to display morphological structures Such as the lamination of the entorhinal cortex. Further, we were able to distinguish between neurons with and Without cyclin B1 expression and we could assign the expression of cyclin B1 to the cell islands of layer II and the pyramidal neurons of layer V of the entorhinal cortex in Alzheimer's disease effected brain. In addition, we developed a method depicting the three-dimensional distribution of the cells in intact tissue sections. Conclusions: In this pilot experiments we could demonstrate the power of the LSC for the analysis of human brain sections. (c) 2006 International Society for Analytical Cytology.