Journal
ANNALS OF THE RHEUMATIC DISEASES
Volume 69, Issue 7, Pages 1396-1402Publisher
BMJ PUBLISHING GROUP
DOI: 10.1136/ard.2009.121400
Keywords
-
Categories
Funding
- Dana Foundation
- Swedish Research Council
- Swedish Society of Medicine
- Swedish Rheumatism Association
- Swedish Cancer Foundation
- Uppsala University Hospital Development Foundation
- Agnes and Mac Rudberg Foundation
- Nilsson Foundation
- King Gustaf V 80-year Foundation
- COMBINE
Ask authors/readers for more resources
Objectives To study the presence of interferogenic autoantibodies in systemic sclerosis (SSc) and their correlation with clinical manifestations, serum levels of interferon alpha (IFN alpha) and chemokines of importance in the disease process. Methods Peripheral blood mononuclear cells (PBMCs) or purified plasmacytoid dendritic cells (pDCs) from healthy donors were stimulated with sera from patients with SSc (n=70) or healthy individuals (n=30), together with necrotic or apoptotic cell material. The IFN alpha produced and serum levels of IFN alpha, IFN-inducible protein-10 (IP-10)/chemokine (C-X-C motif) ligand 10, monocyte chemoattractant protein-1 (MCP-1)/(C-C motif) ligand-2 (CCL-2), macrophage inflammatory protein-1 alpha (MIP-1 alpha)/CCL-3 and RANTES/CCL-5 were measured and correlated with the presence of autoantibodies and clinical manifestations in the patients with SSc. Results Sera from both diffuse SSc and limited SSc contained interferogenic antibodies, which correlated with the presence of anti-ribonucleoprotein and anti-Sjogren syndrome antigen autoantibodies. The pDCs were responsible for the IFN alpha production which required interaction with Fc gamma RII and endocytosis. Increased serum levels of IP-10 were associated with vascular manifestations such as cardiac involvement (p=0.027) and pulmonary arterial hypertension (p=0.036). Increased MCP-1 or IFN alpha serum levels were associated with lung fibrosis (p=0.019 and 0.048, respectively). Digital ulcers including digital loss were associated with increased serum levels of IFN alpha (p=0.029). Conclusion An activated type I IFN system previously seen in several other systemic autoimmune diseases is also present in SSc and may contribute to the vascular pathology and affect the profibrotic process.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available