Journal
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
Volume 384, Issue 6, Pages 1291-1296Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-006-0310-z
Keywords
molecularly imprinted polymer; selective recognition; hydrogen bonding; solvent effect; liquid chromatography
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To clarify the role of diluents in the preparation of molecularly imprinted polymers utilizing only hydrogen bonding, we investigated the effects of diluents by using different solvents. Melatonin (N-acetyl-5-methoxytryptamine), an amide bond and indole ring-containing hormone was chosen as the target molecule. N-Propionyl-5-methoxytryptamine was used as the pseudo template, methacrylic acid as the functional monomer, and solvents were used as diluents. Interactions between the template, the functional monomer, melatonin, and the solvents, were observed by H-1 NMR spectroscopy. The polymers were evaluated by high-performance liquid chromatography. The results suggest the hydrogen bonding-acceptor capacity of the solvent is the most important factor in the preparation of molecularly imprinted polymers for hydrogen bonding-donating molecules. Hydrogen bonding between the template, the functional monomer, and solvent can be estimated from the chemical shifts in H-1 NMR spectra of those molecules in the solvent.
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